3D Cell-Based Biosensors in Drug Discovery Programs
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portes grátis
3D Cell-Based Biosensors in Drug Discovery Programs
Microtissue Engineering for High Throughput Screening
Kisaalita, William S. (University of Georgia, Athens, USA)
Taylor & Francis Ltd
06/2017
404
Mole
Inglês
9781138112025
15 a 20 dias
Descrição não disponível.
Introduction Biosensors and Bioassays Conventional Biosensors Conventional Biosensor Applications Cell-Based Biosensors versus Cell-Based Assays (Bioassays) 3D Cultures Concluding Remarks Target-Driven Drug Discovery Drug Discovery and Development The Taxol (Paclitaxel) Discovery Case The Gleevec (Imatinib Mesylate) Dicovery Case Target-Driven Drug Discovery Paradigm The New Discovery Paradigm Promise Concluding Remarks 3D versus 2D Cultures Comparative Genomics and Proteomics Transcriptional Profi ling Studies Comparative GO Annotation Analysis Proteomics Studies Concluding Remarks Comparative Structure and Function Complex Physiological Relevance Cardiomyocyte Contractility Liver Cell Bile Canaliculi In Vitro Nerve Cell Voltage-Gated Calcium Signaling Concluding Remarks Emerging Design Principles Chemical Microenvironmental Factors Cell Adhesion Molecules Short-Range Chemistry Long-Range Chemistry Concluding Remarks Spatial and Temporal Microenvironmental Factors Nano- and Microstructured Surfaces Scaffolds Nano and Scaffold-Combined Structures Temporal Factor Concluding Remarks Material Physical Property and Force Microenvironmental Factors Basics Stiffness-Dependent Responses Force-Dependent Responses Concluding Remarks Proteomics as a Promising Tool in the Search for 3D Biomarkers Why Search for Three-Dimensionality Biomarkers? Cellular Adhesions Signaling Pathways Overview of Proteomics Techniques Study Design and Methods Concluding Remarks Readout Present and Near Future Readout Present and Near Future Fluorescence-Based Readouts Bioluminescence-Based Readouts Label-Free Biosensor Readouts Concluding Remarks Ready-to-Use Commercial 3D Plates Introduction Algimatrix (TM) Extracel (TM) Ultra-Web (TM) Market Opportunities Concluding Remarks Technology Deployment Challenges and Opportunities Challenges to Adopting 3D Cultures in HTS Programs Typical HTS Laboratory and Assay Configurations Just-in-Time Reagents Provision Model Limited Value-Addition from 3D Culture Physiological Relevance: Transepithelium Drug Transport and Induction of Drug Metabolizing Enzyme Cases Paucity of Conclusive Support of 3D Culture Superiority Cases for 3D Cultures in Drug Discovery Three Cases The ss1-Integrin Monoclonal Antibody Case The Matrix Metalloproteinase Inhibitors Case Resistance to the Chemotherapeutic Agents Case Concluding Remarks Ideal Case Study Design Rationale for The Case Study Why Hepatotoxicity? Hepatotoxicity and hESC-Derived Hepatocyte-Like Cells Study Design and Methods Analysis and Expected Results Appendix A: Patents for 3D Scaffolds Appendix B: Current Drug Targets Appendix C: Popular Cell Lines in Drug Discovery Appendix D: Stem Cells in Drug Discovery Index
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Introduction Biosensors and Bioassays Conventional Biosensors Conventional Biosensor Applications Cell-Based Biosensors versus Cell-Based Assays (Bioassays) 3D Cultures Concluding Remarks Target-Driven Drug Discovery Drug Discovery and Development The Taxol (Paclitaxel) Discovery Case The Gleevec (Imatinib Mesylate) Dicovery Case Target-Driven Drug Discovery Paradigm The New Discovery Paradigm Promise Concluding Remarks 3D versus 2D Cultures Comparative Genomics and Proteomics Transcriptional Profi ling Studies Comparative GO Annotation Analysis Proteomics Studies Concluding Remarks Comparative Structure and Function Complex Physiological Relevance Cardiomyocyte Contractility Liver Cell Bile Canaliculi In Vitro Nerve Cell Voltage-Gated Calcium Signaling Concluding Remarks Emerging Design Principles Chemical Microenvironmental Factors Cell Adhesion Molecules Short-Range Chemistry Long-Range Chemistry Concluding Remarks Spatial and Temporal Microenvironmental Factors Nano- and Microstructured Surfaces Scaffolds Nano and Scaffold-Combined Structures Temporal Factor Concluding Remarks Material Physical Property and Force Microenvironmental Factors Basics Stiffness-Dependent Responses Force-Dependent Responses Concluding Remarks Proteomics as a Promising Tool in the Search for 3D Biomarkers Why Search for Three-Dimensionality Biomarkers? Cellular Adhesions Signaling Pathways Overview of Proteomics Techniques Study Design and Methods Concluding Remarks Readout Present and Near Future Readout Present and Near Future Fluorescence-Based Readouts Bioluminescence-Based Readouts Label-Free Biosensor Readouts Concluding Remarks Ready-to-Use Commercial 3D Plates Introduction Algimatrix (TM) Extracel (TM) Ultra-Web (TM) Market Opportunities Concluding Remarks Technology Deployment Challenges and Opportunities Challenges to Adopting 3D Cultures in HTS Programs Typical HTS Laboratory and Assay Configurations Just-in-Time Reagents Provision Model Limited Value-Addition from 3D Culture Physiological Relevance: Transepithelium Drug Transport and Induction of Drug Metabolizing Enzyme Cases Paucity of Conclusive Support of 3D Culture Superiority Cases for 3D Cultures in Drug Discovery Three Cases The ss1-Integrin Monoclonal Antibody Case The Matrix Metalloproteinase Inhibitors Case Resistance to the Chemotherapeutic Agents Case Concluding Remarks Ideal Case Study Design Rationale for The Case Study Why Hepatotoxicity? Hepatotoxicity and hESC-Derived Hepatocyte-Like Cells Study Design and Methods Analysis and Expected Results Appendix A: Patents for 3D Scaffolds Appendix B: Current Drug Targets Appendix C: Popular Cell Lines in Drug Discovery Appendix D: Stem Cells in Drug Discovery Index
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